موضة

Proteomics Mineathon Challenge update — we’re almost there!

This might possibly be one of my very favorite idea I've had in my life. Current status, we have: 1) A ridiculously cool and important 2) Some impressive impartial judges 3) An almost ready launch page! 4) Like 30+ awesome (and patient) participants!!!! We're reviewing the rules and some final details and we should be good to go. Had no one signed up, it would have launched right on time (probably) but with so many people volunteering their time I owe it to everyone to try and come as close as possible to doing it right.

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Is a peptide quantitatively measurable? Here’s how you find out!

Okay....are you guys ready for this one? I wish I could say I was, but it's too important for us as a field to not think about.... Matrix matching? "Analytical figures of merit"?? Hey! This is the proteomics party, don't you come in here with all your boring analytical chemistry validation stuff....oh.....ugh...okay.... (Yes. I had to make that. You're welcome.) Why is this (study) important? In part because it addresses 2 separate concepts that need to be separated -- and they're right in the abstract: "....Our results demonstrate that increasing the number of detected peptides in a proteomics experiment does not necessarily result in increased numbers of peptides that can be measured quantitatively....." What? First of all, this study is like 4 pages or something and it represents an absurd amount of work. SRMs and DIA experiments (QE HF, I think) and a bunch of different HPLCs and the matrices are all sorts of fun -- CSF and FFPE and yeast digest and maybe I mi..

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The single cell proteomics revolution!

There are seriously 10 papers open on my desktop that I want to blog about -- and will! -- but I'm busy, so time for another super lazy post. Last year some cool people asked me if I'd be interested in doing some articles about things happening in proteomics that I absolutely thought the outside world should know about. My first thought?!?? Single cell proteomics (by SCoPE-MS). This is the best I could come up with. (Of course, I love to type, so I also talked about the study I credit with making proteomics a reality for the rest of us.) On this topic, I recently was so sleepy that I went through all the "comments" on my blog. There was around 2,000 spam messages suggesting all sorts of terribleness, but there were also some legit comments. And -- I tell you what -- SCoPE-MS gets some comments. Particularly regarding aspects of the RAW data in the public repositories, and I think that is something we will really need to talk about at some point. My opinion is that we've ..

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Purple — Pick unique peptides for viral (and other?) experiments from FASTA!

Hey you! Are you looking for a tool to help you select viral peptides for targeted assays? Unrelated --- what is the best color of dinosaur? I got you, yo. Check this out. Before you panic, when they wrote the paper "Purple" was just a Python script that you can get here. I assure you this is no longer the case. There is a very straight-forward (to install) executable that will set you up with a GUI that looks just like this -- -- that you can get here. What does it do? Well, it helps you select peptides that are ideal for targeted assays from the databases you feed it. Imagine Picky, but you can load stuff that isn't human into it. (If you are doing human proteomics -- you should be using Picky, btw. It's amazing). Purple: Feed it your peptide sequences you're interested in: Feed it your contaminating background. Choose your rules. Get your peptides!

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نايضة بين بشرى الضو و دنيا بوطازوت بعد زواجها

على عكس كل من قدم التهاني لدنيا بزواجها الاخير, نشرت بشرى الاعلامية المثيرة للجدل تدوينة على صفحتها الانستغرام تنتقد من خلالها دنيا بشدة حيث قالت خلال تصوير حلقة عيد الحب تلاقيت صدفة بدنيا بوطازوت (الشعيبية) اللي فقدات شعبيتها بعد القضية اياها. السيدة سايكة كات كات ديالها و معفراااا على البشر …

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اكش وان اكن وان كيوجد للعرس ديالو وها أول حاجة غادي ادير

اخر ظهور لاكشوان اكنوان و زوجته رفقة بائع المجوهرات على مائدة الافطار في اجواء مرحة حيث صرح مصطفى انهميقومون بالاستعدادات اللازمة لاحياء الحفل الاسطوري رفقة العديد من الفنانين و قام بشكر كل من سانده و كل من انتقده

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مفاجأة من العيار الثقيل هذا مايحدث امامك دون ان تنتبه له

مفاجأة من العيار الثقيل هذا مايحدث امامك دون ان تنتبه له اكد الخبير المحلي الدكتور زعيترا المتخصص في جراحة الانف والحنجرة واستشاري المسالك البولية على أنه قد نفى أن يكون قد خرج بأي تصريح في السابق حول ما تمت نسبته له الان ـ باعتباره شخصية اعتبارية وغير حقيقة , أي …

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مفاجأة من العيار الثقيل هذا مايحدث امامك دون ان تنتبه له

اكد الخبير المحلي الدكتور زعيترا المتخصص في جراحة الانف والحنجرة واستشاري المسالك البولية على أنه قد نفى أن يكون قد خرج بأي تصريح في السابق حول ما تمت نسبته له الان ـ باعتباره شخصية اعتبارية وغير حقيقة , أي انه لا وجود له على أرض الواقع , وبهذا النفي القاطع …

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القصة الكاملة وراء انتحار متزوجة ببرشيد و غموض حول علاقة مشبوهة

في تصريح صحفي مع حماة الفتاة اثر انتحارها أمس بمدينة برشيد تقول الحماة نا بعد موت جد الفتاة تعرضت لصدمة و دخلت في حالة من الخوف و الوسواس و ظهرت عليها تصرفات غريبة على غير المعتاد حيث طلبت من الحماة الجلوس بمفردها و كأنها على ما يرام . أمس يوم …

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